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Lastly, 1 μCi of 86Rb was included to each culture and incubation was ongoing at 37 °C for a further ten min. Reactions had been terminated by washing the cells with ice-chilly 100 mM MgCl2 and mobile lysates had been geared up for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain were subtracted from those